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pcg backbone  (Addgene inc)


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    Structured Review

    Addgene inc pcg backbone
    Pcg Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcg+plasmid/pCG+(Plasmid+%2351476)/pmc12911861-183-6-8
    Average 93 stars, based on 8 article reviews
    pcg backbone - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A lentiviral vector B cell gene therapy platform for the delivery of the anti-HIV-1 eCD4-Ig-knob-in-hole-reversed immunoadhesin.
    Article Snippet: .. Both glycoproteins were inserted into pCG plasmid driven by the cytomegalovirus early promoter (Addgene #51476). ..

    Article Title: CD46 Null Packaging Cell Line Improves Measles Lentiviral Vector Production and Gene Delivery to Hematopoietic Stem and Progenitor Cells.
    Article Snippet: .. Both glycoproteins were inserted into pCG plasmid driven by the cytomegalovirus early promoter (#51476; Addgene). .. The cytoplasmic tail of the H and F glycoproteins was deleted by truncation of 24 and 30 amino acids (aa), respectively, and has been previously described.17 For use in CD46 null studies, pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid 62988).47 Molecu

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..

    Article Title: A lentiviral vector B cell gene therapy platform for the delivery of the anti-HIV-1 eCD4-Ig-knob-in-hole-reversed immunoadhesin
    Article Snippet: .. Both glycoproteins were inserted into pCG plasmid driven by the cytomegalovirus early promoter (Addgene #51476). ..

    Article Title: CD46 Null Packaging Cell Line Improves Measles Lentiviral Vector Production and Gene Delivery to Hematopoietic Stem and Progenitor Cells
    Article Snippet: .. Both glycoproteins were inserted into pCG plasmid driven by the cytomegalovirus early promoter (#51476; Addgene). ..

    Expressing:

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..

    Construct:

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..

    Generated:

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..

    Cloning:

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..

    Strep-tag:

    Article Title: Stabilized Full-Length Measles Fusion Protein Elicits Potent Immunity and Protection In Vivo
    Article Snippet: A codon-optimized fusion protein sequence derived from the measles virus strain Ichinose-B95a (Taxonomy ID: 645098, Uniprot ID: Q786F3) was synthesized by GenScript and subcloned into the pcDNA3.1 vector. .. The FL expression constructs were generated by cloning the F open reading frame into a pCG plasmid (Addgene ID #84817 ) and adding a C-terminal twin Strep-Tag II using the NEBuilder HiFi DNA Assembly. ..



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